Importance of randomization in microarray experimental designs with Illumina platforms
نویسندگان
چکیده
منابع مشابه
Importance of randomization in microarray experimental designs with Illumina platforms
Measurements of gene expression from microarray experiments are highly dependent on experimental design. Systematic noise can be introduced into the data at numerous steps. On Illumina BeadChips, multiple samples are assayed in an ordered series of arrays. Two experiments were performed using the same samples but different hybridization designs. An experiment confounding genotype with BeadChip ...
متن کاملExperimental comparison and cross-validation of the Affymetrix and Illumina gene expression analysis platforms
The growth in popularity of RNA expression microarrays has been accompanied by concerns about the reliability of the data especially when comparing between different platforms. Here, we present an evaluation of the reproducibility of microarray results using two platforms, Affymetrix GeneChips and Illumina BeadArrays. The study design is based on a dilution series of two human tissues (blood an...
متن کاملUsing lumi, a package processing Illumina Microarray
5 Data preprocessing 3 5.1 Intelligently read the BeadStudio output file . . . . . . . . . . . . 5 5.2 Quality control of the raw data . . . . . . . . . . . . . . . . . . . 8 5.3 Background correction . . . . . . . . . . . . . . . . . . . . . . . . 18 5.4 Variance stabilizing transform . . . . . . . . . . . . . . . . . . . . 19 5.5 Data normalization . . . . . . . . . . . . . . . . . . . . . . ...
متن کاملlumi: a pipeline for processing Illumina microarray
UNLABELLED Illumina microarray is becoming a popular microarray platform. The BeadArray technology from Illumina makes its preprocessing and quality control different from other microarray technologies. Unfortunately, most other analyses have not taken advantage of the unique properties of the BeadArray system, and have just incorporated preprocessing methods originally designed for Affymetrix ...
متن کاملDrosophila microarray platforms.
The advent of high throughput microarrays and the complete sequencing of the Drosophila melanogaster genome have enabled global gene expression analysis in this powerful genetic model organism. Currently, researchers are using three main Drosophila array platform types, with elements composed of cDNA amplicons, oligonucleotides (short and long) or genomic amplicons. This paper provides a broad ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2009
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkp573